spectrofluorimetric determination of cefixime using terbium-danofloxacin probe

Authors

jamshid l manzoori department of analytical chemistry, faculty of chemistry, university of tabriz, tabriz, iran

mohammad amjadi tuberculosis and lung disease research center and faculty of pharmacy, tabriz university of medical sciences, tabriz, iran

naser soltani tuberculosis and lung disease research center and faculty of pharmacy, tabriz university of medical sciences, tabriz, iran

abolghasem jouyban drug applied research center and faculty of pharmacy, tabriz university of medical sciences, tabriz, iran

abstract

objective(s):cefixime (cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. there are some procedures for the determination of cfx in pharmaceutical formulations and biological samples. herein a spectrofluorimetric method was proposed for cfx determination based on the fluorescence quenching of terbium-danofloxacin (tb3+-dano) in the presence of cfx. materials and methods: cfx was detected based on fluorescence quenching of terbium-danofloxacin (tb3+-dano) in the presence of cfx with maximum excitation and emission wavelengths at 347 nm and 545 nm, respectively. the quenched fluorescence intensity of tb3+- dano system is proportional to the concentration of cfx. the optimum conditions for the determination of cfx were studied. results: the maximum response was achieved under optimum conditions of [tris buffer]= 0.008 mol/l (ph 6.5), [tb3+]=1×10-4 mol/l  and [dano]=1×10-4 mol/l. the developed method was evaluated in terms of accuracy, precision and limit of detection. the linear concentration ranges for quantification of cfx were 8.8×10-8-8.8×10-7 mol/l and 1.1×10-7-8.8×10-7 mol/l in standard and human serum samples with the detection limits (s/n=3) of 2.8×10-8 mol/l and 3.9×10-8 mol/l, respectively. the cfx was determined in pharmaceutical tablets and spiked serum samples and the results were satisfactory.   conclusion: this method is simple, practical and relatively interference-free for determination of cfx in pharmaceutical tablets and serum samples.

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Journal title:
iranian journal of basic medical sciences

جلد ۱۷، شماره ۴، صفحات ۲۵۶-۲۶۲

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